Naturally Occurring Flavonol, Quercetagetin 5,6,7,3′,4′-Pentamethyl Ether (Marionol), as a Nontoxic Plant-Based Fluorescent Probe for Rapid, Sensitive, and Selective Detection of Cu2+ in Water

İngilizce (OpenAlex)

High Resolution Image Download MS PowerPoint Slide Herein, we report a naturally occurring flavonol, quercetagetin 5,6,7,3′,4′-pentamethyl ether (known as marionol), as a nontoxic fluorescent probe for rapid, sensitive, and selective detection of Cu 2+ in water. The interaction between marionol and Cu 2+ ions is studied by various state-of-the-art spectroscopic techniques such as 1 H NMR, UV–vis, fluorescence, and high-resolution mass spectrometry (HRMS). Marionol shows strong fluorescence quenching in the presence of Cu 2+, whereas other interfering cations such as Fe 3+, Co 2+, Al 3+, Pb 2+, Hg 2+, Zn 2+, Mg 2+, Mn 2+, Ca 2+, Cu +, Ag +, Na +, and K + produce negligible changes in fluorescence. The binding stoichiometry between marionol and Cu 2+ is found to be 2:1, according to Job’s plot and HRMS analyses. The binding constant ( K a ) of Cu 2+ with marionol is calculated to be 2.89 × 10 9 M –2, and the limit of detection is found to be 0.15 μM. In addition, marionol is further used for the quantification of Cu 2+ in natural spring water samples. The good recoveries (92–110% with RSD <5%) and its low toxicity to MCF-7 human breast cancer cells make marionol a unique plant-based fluorescent probe for the detection of Cu 2+ ions.