Makale detayı · 2024
Investigation of the Effect of Tamoxifen on hERG1 Potassium Channel Gene Expression in MCF-7 Cell Line
Russian Journal of Bioorganic Chemistry
- Yıl
- 2024
- ISSN
1068-1620- Tür
- article
Veri kaynağı ayrımı
- YÖKSİS YÖKSİS makale kaydı
- OpenAlex OpenAlex zenginleştirmesi (özet, atıf, konular)
Özet
İngilizce (OpenAlex)
Abstract Objective: Tamoxifen (TAM) is a nonstreroidal antiestrogenic pharmaceutical agent extensively employed in the therapeutic management of breast cancer. Alterations in the expression patterns of diverse ion channels have been implicated in the pathophysiology of breast cancer. The principal objective of this investigation was to elucidate the impact of TAM on the expression of the hERG1 potassium channel in the MCF-7 cell line. Methods: Cytotoxicity induced by TAM across various concentrations was assessed through the utilization of the MTT method. The patch clamp method was employed for electrophysiological analyses. The gene expression of the hERG1 potassium channel was quantified utilizing RT-qPCR. Results: The electrophysiological findings revealed reduction in potassium channel currents, reaching maximal inhibition at a TAM concentration of 5 μM. Concurrently, hERG1 potassium channel gene expression exhibited a decrease at the 5 μM TAM concentration. Despite statistical analysis, no statistically significant values were identified in both potassium channel currents and gene expression analyses when compared to the control group. Our results demonstrate that the blocking effect of TAM on potassium channel currents in the MCF-7 cell line was not determined. Conclusions: Further investigations are warranted to elucidate comprehensively the role of TAM in modulating hERG1 in breast cancer.
Konular
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
Birincil konu Ion channel regulation and function