Histopathological and Ultrastructural Effects of Glyphosate on the Mantle Tissue of Melanopsis praemorsa (Linnaeus, 1758)

OpenAlex İngilizce

This study aimed to investigate pesticide pollution in freshwater ecosystems and to assess the potential of M. praemorsa as a bioindicator. Study examined the histopathological and ultrastructural changes in the mantle tissue of the freshwater snail Melanopsis praemorsa (Linnaeus, 1758) exposed to sublethal concentrations of glyphosate during a 30-day exposure. The experiment consisted of four groups: a control group and three glyphosate groups (0.933 mg/L, 1.867 mg/L, and 4.666 mg/L). Each group was randomly assigned 30 similarly sized snails. and mantle tissues were sampled on days 10, 20, and 30 of exposure. After fixation/processing, tissues were examined by light microscopy and TEM. Histopathological findings included epithelial desquamation, atrophy of muscle fibers, degeneration of cilia, increased lipid vacuolization, and necrosis. At the ultrastructural level, mitochondrial degeneration, nuclear abnormalities, and cellular lysis were observed. The severity of lesions in the mantle tissue increased depending on glyphosate concentration and exposure duration.