DEVELOPMENT OF IN VITRO STERILIZATION PROTOCOL FOR DO-1 (Prunus domestica) ROOTSTOCK

İngilizce (OpenAlex)

Commercial companies have focused on clonal rootstocks free of diseases and been propagated through tissue culture for fruit seedling production lately to obtain products with higher quality and quantity.Two important stages in tissue culture are surface sterilization and in vitro culturing of the explants to be micro-propagated.Because prunus varieties which sources of explants are grown on open lands, contamination risk is higher.This study has been conducted to minimize the contamination of DO1 (Prunus domestica) explants by microorganisms as prunus rootstock for micropropagation.Several concentrations and application durations of 4 different sterilization agents (HgCl2, NaClO, H2O2, and AgNO3) have been applied on the axillary buds taken from the rootstock for surface sterilization.Then, the explants have been transferred to MS nutrient media and the number of the aseptic explants was determined.Shoot and leaf regeneration have been observed on the aseptic explants 40 days after the application.While the highest aseptic explant rate (91%) was achieved with 2% NaClO application in 15 minutes, the lowest aseptic explant rate (23.33%) was achieved with 0.5% HgCl2 application in ten days.Finally, the lowest contamination rate was observed in NaClO applications (18.34%) and the highest contamination rate was observed in HgCl2 applications among the sterilization agents.