The Effect of Curcumin Supplementation on Irisin, Nesfatin-1, and Leptin Levels in Rats Subjected to Long-Term Treadmill Exercise

İngilizce (OpenAlex)

Background.Curcumin supports metabolism with its antioxidant and antiinflammatory effects.It is particularly effective in reducing metabolic stress caused by prolonged exercise.Objectives.This study aimed to investigate the impact of curcumin supplementation on Irisin, Nesfatin-1, and Leptin hormone levels in rats undergoing prolonged treadmill exercise.Methods.A total of 32 male Wistar-Albino rats were divided randomly into four equal groups: The control group (n=8), the curcumin group (n=8), the treadmill group (n=8), and the curcumin+treadmill group.The experiment lasted eight weeks, with one week of treadmill acclimatization.No intervention was applied to the control group.Curcumin was given at 200 mg/kg, and treadmill exercise was performed for 30 min at 45 cm/s, three days weekly.The Curcumin+Treadmill group was fed 200 mg/kg/day and performed three days a week for 30 minutes at an average speed of 45 cm/s.After the study, rats were euthanized, and blood samples were collected for analysis.Irisin, nesfatin-1, and leptin hormone levels were determined using the ELISA method.Results.Our study showed a significant increase in irisin levels in both the curcumin and exercise groups compared to the control group.For nesfatin-1, levels were significantly decreased in the curcumin group compared to the control, while they were significantly increased in the curcumin+exercise group.Leptin levels were the highest in the exercise group, showing a significant increase compared to the control group (p<0.05).In contrast, the leptin levels in the curcumin group were significantly lower than those in the exercise group.Conclusion.This study demonstrates that combining curcumin supplementation and exercise positively affects irisin, nesfatin-1, and leptin hormone levels, contributing to metabolic balance.