A multidimensional study for design of phytochemical profiling, antioxidant potential, and enzyme inhibition effects of ışgın (Rheum telianum) as an edible plant

OpenAlex · İngilizce

The present study reveals in vitro antioxidant properties and the phytochemical content of a novel Rheum species ( Rheum telianum ) grows and is found in Southeastern Anatolia. To perform the analysis, dried leaves and seeds of the plants were ground and extracted with ethanol to obtain plant secondary metabolites. Then, dried extracts were subjected to in vitro DPPH scavenging and Cupric reducing (CUPRAC), Fe 3+ , and FRAP reducing assays. In addition to the antioxidant capacity assays, quantitative phenolic, flavonoid, and secondary metabolite were determined through LC-MS/MS chromatographic methods. IC 50 values showed that both leaves and the seeds of the R. telianum have high inhibitory properties over DPPH radicals with 20.79 and 5.67 μg/mL, respectively. The samples' dominant secondary metabolites were evaluated using the LC-MS/MS analysis results. The inhibition effects of both leaves and the seeds of the R. telianum extracts acetylcholinesterase and butyrylcholinesterase, α-glycosidase and human carbonic anhydrases II isoenzyme enzymes, which associated with some global diseases including Alzheimer's disease, type-2 diabetes mellitus and glaucoma. In conclusion, the extracts' structure and functional relationship and the plants' possible usage in the food, medicine, and cosmetic industries were made. • Total phenolic and flavonoids in ışgın ( Rheum telianum ) were determined. • Antioxidant activity of ışgın ( Rheum telianum ) was evaluated using several bioassay. • Anticholinergic effect of ışgın ( Rheum telianum ) was determined and compared to Tacrine. • Antidiabetic effect of ışgın ( Rheum telianum ) was realized and compared to Acarbose. • Antiglaucoma effect of ışgın ( Rheum telianum ) was defined and compared to Acetazolamide.