Makale detayı · 2026
Short-Term Bacteriophage Exposure Is Associated with Shifts in Antibiotic Susceptibility Profiles of Clinical Pseudomonas aeruginosa
- Yıl
- 2026
- ISSN
2076-2607- Tür
- article
Veri kaynağı ayrımı
- YÖKSİS YÖKSİS makale kaydı
- OpenAlex OpenAlex zenginleştirmesi (özet, atıf, konular)
Özet
İngilizce (OpenAlex)
Bacteriophage exposure can impose strong selective pressure on bacterial populations and may alter antimicrobial susceptibility beyond direct lytic effects. However, the persistence of such changes after the removal of phage pressure remains insufficiently characterized in clinical P. aeruginosa isolates. This study evaluated whether short-term exposure to the lytic bacteriophage KPP10 was associated with changes in antimicrobial susceptibility categories and whether these changes remained detectable after serial passage in phage-free medium. Five non-duplicate clinical P. aeruginosa isolates were exposed to KPP10 for 24 h at a multiplicity of infection of 10. Antimicrobial susceptibility testing was performed by disk diffusion at baseline, immediately after exposure (F24) and after four serial passages in phage-free medium. Categories were interpreted according to the EUCAST 2024 clinical breakpoints. Nine categorical susceptibility shifts were detected across six antibiotics: four toward increased susceptibility and five toward decreased susceptibility. Six shifts (66.7%) remained detectable after four serial passages, whereas three shifts (33.3%), involving ciprofloxacin, cefepime, and aztreonam in LBK20, reverted to their baseline categories. Eight of the nine shifts involved β-lactam antibiotics, and cefepime was the most frequently affected agent. These exploratory findings show that short-term KPP10 exposure was associated with isolate-specific and bidirectional changes in categorical antimicrobial susceptibility and support repeated susceptibility monitoring in future phage–antibiotic studies.
Konular
- Bacteriophages and microbial interactions
- Antibiotic Use and Resistance
- Antibiotic Resistance in Bacteria
Birincil konu Bacteriophages and microbial interactions