Effect of Thiocolchicoside on Midline Closure in Early Chicken Embryos

İngilizce (OpenAlex)

BACKGROUND/AIMS: Thiocolchicoside (TCC) is a semi-synthetic derivative of colchicine analog, a muscle relaxant with analgesic and antiinflammatory activity.Studies have shown that colchicine inhibits microtubule polymerization and stops mitotic activity, and that TCC is a competitive antagonist of g-aminobutyric acid A and glycine receptors.It is known that the use of TCC during pregnancy may have teratogenic effects.We aimed to examine the effects of drugs with TCC as their active ingredient, which are frequently used in clinical practice, on the development of chicken embryos and midline closure. MATERIALS AND METHODS:A total of 80 eggs were incubated in an incubator for 24 hours.At the 24 th hour of incubation, the eggs were divided into 4 groups.Increasing doses of TCC (8 mcg, 16 mcg, 32 mcg) in 0.1 cc solutions were applied to these groups and half of the control group was administered physiological saline, corresponding to the air sac.All eggs were then closed with sterile tapes and replaced in the incubator.On the 10 th day of incubation, the eggs were hatched and the embryos were evaluated morphologically and histopathologically. RESULTS:TCC caused a high rate of early embryo death (EED) in all groups in which it was applied.Although midline closure defects were detected in some of the developing embryos, it was not statistically significant.CONCLUSION: EED and midline closure defects were observed in some developing embryos in the TCC applied groups.It will be possible to understand the mechanism of embryonic damage, to reveal teratogenic effects and to minimize the formation of congenital defects with more comprehensive studies.