Makale detayı · 2003
Immobilization of Phospholipase A2 on porous glass and its application for lowering serum cholesterol concentration
Artificial Cells, Blood Substitutes and Immobilization Biotechnology
- Yıl
- 2003
- ISSN
1073-1199- Tür
- article
Veri kaynağı ayrımı
- YÖKSİS YÖKSİS makale kaydı
- OpenAlex OpenAlex zenginleştirmesi (özet, atıf, konular)
Özet
İngilizce (OpenAlex)
Phospholipase A2 (PLA2; EC 3.1.1.4) is a lipolytic enzyme that hydrolysis the ester bond in sn-2 position of phospholipids. In this work, the PLA2 from hog pancreas was covalently coupled to porous glass. The properties of free and immobilized enzyme were also investigated and compared. The optimum pH and temperature were found as 8.5 and 50 degrees C, respectively for both free and immobilized enzyme. The immobilized enzyme had good properties that potential for medical application is considerable. Its use in lowering plasma cholesterol concentrations in blood samples was also demonstrated.
Konular
- Enzyme Catalysis and Immobilization
- Advanced Proteomics Techniques and Applications
- Electrochemical sensors and biosensors
Birincil konu Enzyme Catalysis and Immobilization