EVALUATION OF PHARMACEUTICAL POTENTIALS OF NATURALLY AND IN VITRO-GROWN FRAGARIA VESCA L (WILD STRAWBERRY)

OpenAlex İngilizce

Wild strawberry (Fragaria vesca L.) is a perennial herb of the Rosaceae family that grows a rosette of leaves from a central rootstock. It has been used in traditional medicine to treat digestive disorders, inflammation, and skin diseases, as well as for its nutritional value and pleasant flavor. This plant has attracted the interest of the pharmaceutical sector due to its medicinal properties. The objective of this study was to screen and compare the pharmaceutical potentials of various extracts of F. vesca obtained from field-grown leaves and fruits, and in vitro-grown leaves and callus using specific bioassays (antibacterial, antioxidant, antitumor, toxicity and anticancer) with different extraction methods. Field-grown leaves and fruits revealed better antibacterial activity than in vitro-grown leaves and callus. E. cloacae was the most vulnerable to the antibacterial activity of the field grown extracts. Field grown-leaves showed the highest antioxidant activity and total phenol-flavonoid content among the tested extracts. Potato disc tumor induction assay revealed that aqueous and methanolic extracts of field-grown leaves, as well as a hot ethanolic extract of fresh fruit, had the highest anti-tumor activity. In the brine shrimp toxicity test, extracts of field-grown leaves and fruits were more toxic than extracts of in vitro-grown leaves and callus. Extracts of field-grown leaves and fruits had the strongest anticancer action on Human Breast Adenocarcinoma (MCF-7) cell lines. Our findings have revealed that naturally grown-F. vesca leaves were the most efficient in terms of pharmaceutical potential.