Expression of miRNAs in prostate cancer cell lines and prostate epithelial cell lines

İngilizce (OpenAlex)

Expression of miRNAs in prostate cancer cell lines and prostate epithelial cell lines P rostate cancer (PCa) is one of the most common non-skin malignancies and among the main causes of cancer-related death worldwide [1].Globally, more than 650,000 men are diagnosed with PCa each year, accounting for approxi-mately10% of all new cancer cases in men.PCa is the most common solid tissue cancer among men in Western societies.Although the widespread use of the prostate-specific antigen (PSA) test and screening methods have increased the frequency of diagnosis, they have significantly reduced PCa-related metastases and mortality.Several risk factors have been identified in relation to the progression of the disease, but relapse is still reported in up to 30% of PCa patients after radical prostatectomy.Therefore, the molecular mechanisms underlying prostate carcinogenesis must be characterized in order to improve patient survival and the efficacy of available treatment options [1,2].Objectives: Prostate cancer (PCa) is among the most frequently diagnosed cancers and a leading cause of cancer-related deaths in men.Although prostate-specific antigen (PSA) testing has become routine in screening and early detection, PSA has high false-positive rates and poorly correlates with disease stage.Consequently, other diagnostic and prognostic markers for PCa are urgently needed.MicroRNAs (miRNAs) modulate gene expression at the transcriptional level and are known to play key roles in various physiological events.Growing evidence indicates that miRNA dysregulation is involved in the initiation and progression of many diseases, including PCa.Various miRNAs have been associated with PCa pathogenesis, suggesting that miRNA expression profiles have potential utility in the prognosis, diagnosis, and treatment of this disease.miRNA expression levels have been investigated in prostate epithelial cells by PCa cell culture.Methods: The present study compared the expression levels of selected prognostic miRNAs targeting that have been implicated in the pathogenesis of PCa.Using cDNA obtained from the C4-2 human PCa and PNT1a normal prostate epithelial cell lines, miRNA expression levels were quantitatively analyzed via melting curve analysis using the miScript SYBR Green kit in a Rotor-Gene Q real-time polymerase chain reaction (PCR) device.Results: Reverse transcription quantitative PCR analyses have demonstrated miRNA expression levels in the C4-2 PCa cell line and PNT1a prostate epithelial cell line.were found to be overexpressed in the PCa cell line (p<0.05),while there were no statistically significant differences in miR-32 expression of between PCa cells and PNT1a cells (p>0.05).Conclusion: Considering the variation in expression level of miRNAs targeting genes responsible for the etiopathogenesis of PCa.Provides information on the use of miRNAs as prognostic markers in PCa.