Makale detayı · 2022
A New Affinity Matrixe Synthesized from Aminobenzohydrazide Derivatives for Purification of Lactoperoxidase Enzyme
- Yıl
- 2022
- ISSN
2365-6549- Tür
- article
Veri kaynağı ayrımı
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Özet
İngilizce (OpenAlex)
Abstract In this study, a new affinity process was developed for the purification of Lactoperoxidase with synthesized sixteen aminobenzohydrazide derivatives. For this purpose, ligands were covalently bound to CNBr‐activated Sepharose‐4B−L‐tyrosine matrix and affinity columns were prepared, and LPO was purified in one step with high yield and purity. Among all synthesized molecules, the 4‐amino‐3‐bromo‐2‐methylbenzohydrazide molecule had a high usable potential in the purification of Lactoperoxidase from mammalian milk. Lactoperoxidase was purified 411.8 times with a yield of 17.38 % from goat milk, 187.25 times with a yield of 9.72 % buffalo milk, 2772.4 times with a yield of 18.98 % from bovine milk, and 1246.65 times with a yield of 4.43 % from sheep milk. It was demonstrated for the first time that aminobenzohydrazide molecules could be used as ligands in the purification of Lactoperoxidase enzyme.
Konular
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nanoparticles: synthesis and applications
- Enzyme function and inhibition
Birincil konu Neutrophil, Myeloperoxidase and Oxidative Mechanisms