Unveiling the role of miRNA in laryngeal squamous cell carcinoma progression: a retrospective study

OpenAlex İngilizce

OBJECTIVE: MicroRNAs (miRNAs) have emerged as promising biomarkers for diagnosis and prognosis in laryngeal squamous cell carcinoma (LSCC). This study investigates the expression patterns of specific cancer-associated miRNAs in early and advanced stage LSCC to evaluate their potential role in disease progression. METHODS: The expression levels of miRNA-21, miRNA-802, miRNA-29 and miRNA-9 were analysed in formalin-fixed paraffin-embedded (FFPE) tissues from 44 LSCC patients (20 early stage, 24 advanced stage) using quantitative multiplex RT-PCR. Tissue samples were analysed retrospectively. RESULTS: The expression level of hsa-miR-9a expression was significantly upregulated (5.2-fold) in advanced stage patients compared to the early stage group (p = 0.0088). Similarly, hsa-miR-802a showed significantly higher expression (9.2-fold) in advanced stage patients compared to early stage patients (p = 0.005). No significant differences in miRNA-21 and miRNA-29 expression levels were observed between the groups. CONCLUSION: As a result of the study, elevated miRNA levels in advanced stage patients can be used in targeted therapy and evaluated as a treatment option in LSCC patients.