Effect of curcumin on erythrocyte deformability in mice with limb ischemia-reperfusion injury

OpenAlex İngilizce

Objectives: This study aims to examine the impact of curcumin on erythrocyte deformability in a mouse model that can be used for short-term ischemia/reperfusion (IR) injury. Materials and methods:A total of 30 mice were divided into five groups of six animals in each.The mice were subjected to unilateral hind limb ischemia on the femoral vessels for 2 h.Reperfusion was achieved for the next 2 h.Curcumin (100 mg•kg -1 ) was administered intraperitoneally (CUR and IR/CUR groups).The mice were euthanized by intraperitoneal administration of ketamine (100 mg•kg -1 ) and blood was drawn from the heart.A constant flow filter system was used to assess erythrocyte deformability.Results: There was a statistically significant difference in the mean deformability index among the groups.The IR group (2.1±0.3)significantly higher than the mean of control group (1.6±0.2),CUR group (1.6±0.1) and DMSO group (1.6±0.2).The deformability index values of the IR/CUR group approach the control group through the application of the curcumin (1.8±0.2). Conclusion:Our study showed negative effects of IR on erythrocyte deformability in mice.Also, curcumin had positive effects by reversing the undesirable effects of IR.