The protease inhibitor antipain has a beneficial synergistic effect with trehalose for ram semen cryopreservation

İngilizce (OpenAlex)

Contents The objective of this study was to examine the different concentrations of antipain and trehalose combination on post‐thawed quality of ram semen cryopreserved in tris extender. Ejaculates were collected from four rams using the artificial vagina, pooled at 37°C and diluted with (A 0 Tre 0 : antipain 0 μM and trehalose 0 mM (Control); A 10 Tre 0 ; A 50 Tre 0 ; A 0 Tre 30 ; A 0 Tre 60 ; A 10 Tre 60 ; A 10 Tre 30 ; A 50 Tre 30 and A 50 Tre 60 ). Diluted semen samples were gradually cooled down from 37 to 5°C in a cold cabinet; then, they were loaded into 0.25 ml straws, frozen and stored in liquid nitrogen. Sperm motility ( CASA ), viability, membrane functionality and abnormality were evaluated after thawing process. Progressive motility in extender supplemented with A 10 Tre 0 , A 0 Tre 30 and A 10 Tre 60 significantly ( p < 0.05) higher as compared to the control (A 10 Tre 0 ). A 10 Tre 60 (47.50 ± 0.73) provided the best maintenance of progressive motility in comparison with the control (40.50 ± 0.73). No significant differences were observed between all treated groups in terms of total motility, VAP , VSL , VCL , ALH , BCF , STR and LIN . The percentages of sperm with viable were significantly higher in extenders supplemented with A 10 Tre 0 , A 50 Tre 0 , A 0 Tre 30 and A 10 Tre 60 , compared to control. Addition of A 10 Tre 0 , A 50 Tre 0 and A 10 Tre 60 to extenders improved the percentages of sperm abnormality, compared to the controls. A 10 Tre 60 (67.84 ± 1.51) treatment provided the best maintenance of normal morphology compared to the other treatments. The supplementation with A 10 Tre 0 , A 0 Tre 60 and A 10 Tre 60 improved the percentage of sperm membrane functionality when compared to the control ( p < 0.05). Comparing these results with those of control diluents, the effects of supplementation were better except for A 50 Tre 60 group. In conclusion, when combination of antipain (10 μM) and trehalose (30 and 60 mM ) was added, they conferred a great cryosurvival capacity with their synergic effects during freeze‐thawing process.