Pathogenicity of Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) and crude secondary metabolites against Dendroctonus micans (Kugelann,1974) (Coleoptera: Curculionidae)

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Beauveria bassiana (Balsamo-Vuillemin, 1912) (Hypocreales: Clavicipitaceae) is a valuable source of natural bioactive compounds, particularly secondary metabolites. This study, conducted in 2024 at Trabzon Forest Pests and Biological Control Laboratory, aimed to evaluate the insecticidal efficacy of a crude ethyl acetate extract of its secondary metabolites against Dendroctonus micans (Kugelann, 1794) (Coleoptera: Curculionidae) larvae and its antimicrobial activities. Pa-4 strain caused 100% mortality of D. micans larvae within ten days of 1 × 109 spores/mL, and the LC50 value was determined to be 1.63 × 105 spores/mL. The crude secondary metabolites extract concentration was 0.065 g/mL in the biomass and insoluble residues (mycelial extract) and 0.68 g/mL in the supernatant. The LC50 value for micelle-extract was 1019 ppm, while for the supernatant extract it was 1382 ppm (p