RP-HPLC AND HPTLC-BASED PHYTOCHEMICAL STUDIES OF ENDEMIC NEPETA CADMEA BOISS. AND ITS EFFECTS ON CARBOHYDRATE DIGESTIVE ENZYMES

İngilizce (OpenAlex)

This study investigated the in vitro inhibitory potential of the methanol extract of the aerial parts of Nepeta cadmea on α-glucosidase and α-amylase, which are known as key enzymes related to type 2 diabetes mellitus. To determine the active constituents of the endemic Lamiaceae plant, validated RP-HPLC and HPTLC techniques were used. The methanol extract demonstrated moderate and dose-dependent inhibitory activity, with inhibition rates of 43.57 ± 4.32% against α-glucosidase at 2 mg/mL and 36.74 ± 7.23% against α-amylase at 1 mg/mL. Phytochemical analysis revealed that rosmarinic acid (0.6290 ± 0.0095 g/100g dw) was the predominant phenolic compound, followed by chlorogenic acid (0.0429 ± 0.0012 g/100g dw) and caffeic acid (0.0027 ± 0.0002 g/100g dw). These findings suggest that this endemic Lamiaceae plant may have potential as a natural therapeutic agent for managing diabetes mellitus. Further studies are warranted to explore its mechanisms of action and to evaluate its efficacy in clinical settings.